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. 2021 Mar 5;11(10):4975–4991. doi: 10.7150/thno.55074

Figure 4.

Figure 4

FOSL2 is regulated by the estrogen/cAMP/PKA signaling axis. (A) The mRNA levels of CYP19A1 were determined by qRT-PCR in CAFs and NFs. Gene expression was normalized by β-actin (**p<0.01). (.B) Testosterone (100 nM) was added to the culture medium of breast NFs and CAFs. E2 concentrations were determined by chemiluminescence immunoassay (**p<0.01). (C) The production of cAMP in NFs and CAFs was detected by an ELISA Kit (R&D System, USA). Production of cAMP was increased under treatment with E2 (100 nM) in NFs (**p<0.01). (D-F) Protein levels of FOSL2 were detected in CAFs stimulated with SQ22536 (100 μM) (D) and H89 (5 μM) (E) or NFs treated with E2 (100 nM) (F) for 12 h. (G) CYP19A1 levels were detected by qRT-PCR in CAFs transfected with CYP19A1 siRNA (siCYP19A1) and control cells (**p<0.01). (H, I) FOSL2 levels were evaluated by qRT-PCR (H) and western blotting (I) analysis in the indicated cells (*p<0.05, **p<0.01).