Table 1.
Antibody | Species | Target Species | Dilution Ratio | Supplier | Catelogue Number |
---|---|---|---|---|---|
CX3CL1 | Rbt | H, M, R | 1:1000 | Abcam | ab25088 |
CX3CR1 | Rbt | H, M, R | 1:1000 | Abcam | ab8021 |
ICAM-1 | Rbt | H | 1:1000 | Abcam | ab53013 |
LFA-1 | Rbt | H | 1:1000 | CST | no. 73663 |
Src | Rbt | H, M, R | 1:1000 | CST | no. 2108 |
p-Src (Tyr416) | Rbt | H, M, R | 1:1000 | CST | no. 6943 |
Akt | Rbt | H, M, R | 1:1000 | CST | no. 4691 |
p-Akt (Ser473) | Rbt | H, M, R | 1:1000 | CST | no. 4060 |
PI3K | Rbt | H, M, R | 1:1000 | CST | no. 4249 |
p-PI3K (Ser249) | Rbt | H | 1:1000 | CST | no. 13857 |
Vimentin | Rbt | H, M, R | 1:1000 | CST | no. 5741 |
E-cadherin | Rbt | H, M | 1:1000 | CST | no. 3195 |
MMP-2 | Rbt | H | 1:1000 | CST | no. 13132 |
MMP-9 | M | H, M, R | 1:1000 | SC | sc-393859 |
GEF-H1 | Rbt | H, M, R | 1:1000 | CST | no. 4076 |
ROCK1 | Rbt | H, M, R | 1:1000 | CST | no. 4035 |
RhoA | Rbt | H, M, R | 1:1000 | Abcam | ab187027 |
p-RhoA (Ser188) | Rbt | H | 1:1000 | Abcam | ab41435 |
MLC | Rbt | H, M, R | 1:1000 | CST | no. 3672 |
p-MLC (Ser19) | Rbt | H, M, R | 1:1000 | CST | no. 3671 |
MAPK14 | M | H, M, R | 1:1000 | Abcam | ab31828 |
p‑MAPK14 (Tyr180) | Rbt | H | 1:1000 | Abcam | ab4822 |
ADAM17 | Rbt | H, M, R | 1:1000 | Abcam | ab2051 |
p‑ADAM17(Tyr735) | Rbt | H, M, R | 1:1000 | Abcam | ab182630 |
NF‑κB | Rbt | H, M, R | 1:1000 | Abcam | ab16502 |
p-NF‑κB (Ser536) | Rbt | H, M, R | 1:1000 | CST | no. 3033 |
β‑actin | M | H, M, R | 1:1000 | CST | no. 3700 |
GAPDH | Rbt | H, M, R | 1:1000 | Beyotime | no. AF1186 |
Anti-Rabbit IgG | Goat | Rbt | 1:5000 | YEASEN | no. 33101ES60 |
Anti-Mouse IgG | Goat | M | 1:5000 | YEASEN | no. 33201ES60 |
Abbreviations: H, human; Rbt, rabbit; R, rat; M, mouse; CST, Cell Signaling Technology (Danvers, MA, USA); SC, SANTA CRUZ Biotechnology (Dallas, Texas, USA); Beyotime, Beyotime Institute of Biotechnology (Shanghai, China); YEASEN, YEASEN Biotech (Shanghai, China); R&D, R&D SYSTEMS (Minneapolis, MN, USA).