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. Author manuscript; available in PMC: 2021 Nov 18.
Published in final edited form as: Bioconjug Chem. 2020 Nov 4;31(11):2465–2475. doi: 10.1021/acs.bioconjchem.0c00529

Figure 1.

Figure 1.

(a) Schematic for incorporating ketone groups on a cell surface using a glycan metabolic pathway for Staudinger ligation with biotinylated hydrazides for detection with FITC-avidin. (b) Flow cytometry of Jurkat cells reveal that the addition of azido sugars (ManLev-Bio+Av) increase the fluorescence 30-fold as compared to the controls [18]. Reproduced with permission from Mahal, L. K.; Yarema, K. J.; Bertozzi, C. R. Engineering Chemical Reactivity on Cell Surfaces through Oligosaccharide Biosynthesis. Science 1997, 276 (5315), 1125–1128.