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. Author manuscript; available in PMC: 2021 Nov 18.
Published in final edited form as: Bioconjug Chem. 2020 Nov 4;31(11):2465–2475. doi: 10.1021/acs.bioconjchem.0c00529

Figure 2.

Figure 2.

(a) Strategies for cell labeling with azides using metabolic expression of non-natural sugars, followed by Cu-catalyzed click chemistry labeling [21]. (b) Cu-free methods of cell labeling with strained cyclooctynes [23]. Reproduced with permission from: Baskin, J. M.; Prescher, J. A.; Laughlin, S. T.; Agard, N. J.; Chang, P. V.; Miller, I. A.; Lo, A.; Codelli, J. A.; Bertozzi, C. R. Copper-Free Click Chemistry for Dynamic in Vivo Imaging. Proc. Natl. Acad. Sci. U. S. A. 2007, 104 (43), 16793–16797. (c) Comparison of Jurkat cell labeling with a biarylazacyclooctyne (BARAC), fluorinated cyclooctyne (DIFO), and non-fluorinated cyclooctyne (DBCO) [26].