Table 2. Formulation of FLR-DNA-MNP Nanoparticles for In Vitro Magnetofection^a.

MNPs (μg/μg DNA)	DNA (μg)	FLR-DNAb incubation time (min)	OptiMEM (μL)c	MNPs (μL of 10 mg/mL stock)d	FLR/DNA + MNP incubation time (min)	media per well (μL)	total transfection volume (media + OptiMEM) (μL)
5	0.5	15	2 × 12.5	0.25	15	100	125
10	0.5	15	2 × 12.5	0.5	15	100	125
25	0.5	15	2 × 12.5	1.25	15	100	125
50	0.5	15	2 × 12.5	2.5	15	100	125

^aFLR-DNA-MNP complexation for 0.5 μg of DNA per transfection on a 48-well plate format. Scalable to other well plate formats.

^bThe amount of FLR added was adjusted according to the N/P ratio (Table 1).

^cFLR was diluted to a total volume of 12.5 μL in OptiMEM. DNA was diluted to a total volume of 12.5 μL in OptiMEM. DNA solution was added to FLR solution and mixed thoroughly to facilitate particle formation.

^dAn MNP volume was added straight into the FLR-DNA solution and mixed thoroughly.