Table 1. Binding affinities and thermodynamic parameters of peptides/chimeras for hASF1A determined by ITC measurements and NMR spectroscopy.

Peptide/chimera	Kd (μM) *,† (ITC)	N*	ΔH* (kcal/mol)	−TΔS* (kCal/mol)	Kd (μM)‡ (NMR)
P1	0.13 ± 0.04	1.0 ± 0.1	−11.7 ± 0.4	2.9 ± 0.4	ND
P2	0.44 ± 0.02	1.1 ± 0.2	−10.3 ± 0.6	2.2 ± 0.6	ND
P3	0.27 ± 0.07	1.0 ± 0.1	−10.5 ± 0.8	2.2 ± 0.9	ND
P4	0.20 ± 0.09	1.0 ± 0.1	−9.2 ± 0.4	0.7 ± 0.6	ND
C1	10.6 ± 3.8	1.2 ± 0.2	−3.6 ± 0.5	−2.7 ± 0.7	18 ± 6
C2	2.7 ± 0.6	1.0 ± 0.1	−2.2 ± 0.1	−4.8 ± 0.2	4 ± 1
C3	4.6 ± 0.3	1.0 ± 0.1	−3.3 ± 0.1	−3.5 ± 0.7	6 ± 3
C4	13.3 ± 3.5	1.1 ± 0.2	−1.8 ± 0.4	−4.7 ± 0.5	15 ± 7
C5	6.6 ± 4.5	1.1 ± 0.1	−3.5 ± 1.4	−3.1 ± 0.2	ND
C6	4.3 ± 1.4	1.2 ± 0.1	−2.2 ± 0.3	−4.6 ± 0.4	8 ± 3
C7	15.4 ± 4.0	1.4 ± 0.2	−1.7 ± 0.2	−4.4 ± 0.4	16 ± 6
C8	ND	ND	ND	ND	152 ± 90
C9	7.9 ± 0.3	1.2 ± 0.3	−3.5 ± 0.6	−3.0 ± 0.5	7 ± 4
C10	13.1 ± 4.1	1.2 ± 0.2	−1.9 ± 0.4	−4.3 ± 0.2	18 ± 4

*ND, not determined.

†Binding curves are shown in fig. S2.

‡Binding curves are shown in fig. S5.