Fig. 6. SARS-CoV-2 vaccine-induced T cell responses in BALB/c mice.
Splenocytes from mice (n = 5 in each group), immunized with S2PECTO spike, S2GΔHR2 E2p SApNP, and S2GΔHR2 I3-01v9 SApNP, were isolated at w11 and cultured in the presence of IL-2 and dendritic cells (DCs) pulsed with S2PECTO (1 × 10−7 μM), S2GΔHR2 E2p (1 × 10−7 μM), and S2GΔHR2 I3-01v9 (1 × 10−7 μM), respectively. Splenocytes from five naïve mice were used as control samples and cultured in the presence of DCs without antigen pulsing. Cells were assessed after 16 hours (A and C) and 4 hours (B and D) of culture. (A and B) Vaccine-induced CD4+ T cell immunity. (C and D) Vaccine-induced CD8+ T cell immunity. Plots show the frequencies of cell fraction. The P values were determined by one-way ANOVA analysis. *P < 0.05, **P < 0.01, and ***P < 0.001.