Fig. 4. Relationship between resting activity and increases in power and C.C.

(A) Scatterplot of the relative increase in neuronal pCa²⁺ for each neuron during inflammatory pain, plotted against the basal pCa²⁺ before pain, and shown at four different days postoperation (n = 97 neurons per seven mice, Pearson’s correlation test). (B) Comparison of C.C. of paired neurons in control and inflammatory pain model mice (day 3 after CFA injection). In the center and right columns, the data were stratified into neurons with low-basal pCa²⁺ and higher pCa²⁺ (using median pCa²⁺ as the cutoff). A similar difference in C.C. was seen in both these cohorts. ***P < 0.001, Mann-Whitney U test. Violin plots show median (black lines) and distribution of the data. (C) Scatterplots of pain behavioral responses (mechanical threshold or escape response latency during thermal stimulation) against the neuronal C.C. or pCa²⁺. A significant negative correlation existed for C.C. against pain behaviors but not for pCa²⁺. There was no relationship between C.C. and pCa²⁺. Data from pre- and post-CFA neuronal responses, as shown in Fig. 2 [n = 7 mice for (C), regression lines show by Pearson’s correlation test].