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. 2021 Mar 10;47(5):78. doi: 10.3892/ijmm.2021.4911

Figure 3.

Figure 3

Knockdown of LincIN inhibits ESCC cell growth, migration and invasion. (A) Expression of LincIN in LincIN stably depleted and control Eca-109 cells detected by RT-qPCR. Scrambled shRNA targeting nothing was used as a control. (B) Cell growth of LincIN stably depleted and control Eca-109 cells detected by CCK-8 assays. (C) Cell growth of LincIN stably depleted and control Eca-109 cells detected by EdU incorporation assays. Red color indicates EdU-positive cells. Scale bars, 100 µm. (D) Cell migration of LincIN stably depleted and control Eca-109 cells detected by Transwell migration assays. Scale bars, 100 µm. (E) Cell invasion of LincIN stably depleted and control Eca-109 cells detected by Transwell invasion assays. Scale bars, 100 µm. Results are shown as the means ± SD from 3 separate experiments. **P<0.01, ***P<0.001, vs. control-shRNA group determined by one-way ANOVA followed by Dunnett's multiple comparisons test. ESCC, esophageal squamous cell carcinoma.