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. 2021 Mar 11;2021:6669352. doi: 10.1155/2021/6669352

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Copyright © 2021 Nevena Grdović et al.

This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

PMC Copyright notice

The effect of ALA on collagen deposition and Col4a1 gene expression in kidneys of the control and diabetic rats, 4 and 8 weeks after diabetes induction. (a) Masson trichrome staining showing collagen deposition in kidney sections (blue: collagen, pink/violet: cytoplasm, and brown: nuclei). The results of quantification by ImageJ analysis are presented as mean values ± SD. (b) mRNA levels of Col4a1 expression in the kidneys, as determined by RT-qPCR. mRNA levels are relative to Actb. “^∗” indicates statistically significant difference compared to the control group (^∗p < 0.05, ^∗∗p < 0.01, and ^∗∗∗p < 0.001), “^#” indicates statistically significant difference compared to the ALA group (^#p < 0.05, ^##p < 0.01, and ^###p < 0.001).