Figure 6.

The influence of ALA treatment on DNA methylation status of the Col4a1/Col4a2 promoter region. (a) Schematic representation of genomic loci that encompasses Col4a1 and Col4a2 genes and enlarged region of their bidirectional promoter (130 bp long) with marked position of Col4a1 transcription start site (+1), positions of primers used for methylation-specific PCR, and transcription factors binding sites. (b) DNA methylation levels of the selected region of Col4a1/Col4a2 promoter obtained by MSP and estimated as DNA methylation index. ^∗p < 0.05 compared to the control group of animals.