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. Author manuscript; available in PMC: 2022 Mar 17.
Published in final edited form as: Neuron. 2021 Feb 1;109(6):997–1012.e9. doi: 10.1016/j.neuron.2021.01.003

Figure 3. Recruitment of Sncg INs during spontaneous behavior.

Figure 3.

A. Schematic of the experimental design for in vivo calcium imaging of Sncg IN activity with correlated detection of SWRs. Asterisk shows the same event on panels A and B. Voltage traces are shown in black, filtered trace in blue, envelope in orange, horizontal lines depict thresholds (3 and 5 SD, see Methods). T = 0 (dashed line) is where the envelope crosses the first threshold.

B. Example trace of in vivo calcium imaging of a single representative Sncg IN. Vertical lines show the onset of SWR events detected in the contralateral CA1.

C. Event-triggered average activity of individual Sncg INs aligned on Run – Stop and SWR events. Cells are sorted by response magnitude. All recordings were made in awake mice, SWRs were analyzed during rest. Note the different time scale and dynamic range for the two plots. T = 0 is the time of run-stop (treadmill speed drops to zero, top) or SWR onset (bottom).

D. The run- and SWR response scores of Sncg INs. Data was pooled from both experiments shown on this figure and Fig. 4. No effect of experimental group or sex was detected on the run response (likelihood ratio test, p(group) = 0.91, p(sex) = .054, n = 141 cells, 28 sessions, 8 animals, 5 females and 3 males) or the SWR response (p(group) = .11, p(sex) = .20).