Kv3.3 KO or Kv3.3G592R KI inhibits slow endocytosis at calyces
(A) Sampled ICa and mean Cm trace (mean + s.e.m) induced by depol20ms (arrow) from WT (10 calyces, 10 mice), Kv.3.3−/− (10 calyces, 10 mice) and Kv3.3G592R (10 calyces, 10 mice) calyces from P7–10 mice at 22–24°C. s.e.m. is plotted every 1 s.
(B) Ratedecay, ΔCm and QICa (mean + s.e.m.) induced by depol20ms at WT (10 calyces, 10 mice, black), Kv.3.3−/− (10 calyces, 10 mice, red) and Kv3.3G592R (10 calyces, 10 mice, blue) calyces from P7–10 mice at 22–24°C. **: p < 0.01 (t test).
(C) The Cm change (mean + s.e.m.) and Ratedecay (mean + s.e.m.) induced by depol20ms (arrow) at WT (7 calyces, 7 mice) and Kv3.3G592R (7 calyces, 7 mice) calyces from P7–10 mice at 34–37°C. **: p < 0.01 (t test).
(D) Similar to C, except from P13–14 mice at 22–24°C. WT: 8 calyces, 8 mice; Kv3.3G592R: 7 calyces, 7 mice.