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. Author manuscript; available in PMC: 2022 Mar 17.
Published in final edited form as: Neuron. 2021 Jan 27;109(6):938–946.e5. doi: 10.1016/j.neuron.2021.01.006

Figure 2.

Figure 2.

Kv3.3 KO or Kv3.3G592R KI inhibits rapid endocytosis at calyces

(A) Cm traces (mean + s.e.m, every 1 s) induced by depol20msX10 (arrow) in WT (10 calyces, 10 mice), Kv.3.3−/− (10 calyces, 10 mice) and Kv3.3G592R (10 calyces, 10 mice) calyces of P7–10 mice at 22–24°C.

(B) Ratedecay, ΔCm and QICa (mean + s.e.m.) induced by depol20msX10 (arrow) at WT (10 calyces, 10 mice), Kv.3.3−/− (10 calyces, 10 mice) and Kv3.3G592R (10 calyces, 10 mice) calyces from P7–10 mice at 22–24°C. *: p < 0.05; **: p < 0.01 (t test).

(C) The Cm change (mean + s.e.m.) and Ratedecay (mean + s.e.m.) induced by depol20msX10 (arrow) at WT (7 calyces, 7 mice) or Kv3.3G592R (6 calyces, 6 mice, blue) calyces of P7–10 mice at 34–37°C. **: p < 0.01 (t test).

(D) Similar to C, except from P13–14 mice at 22–24°C. WT: 8 calyces, 8 mice; Kv3.3G592R: 7 calyces, 7 mice.

(E-F) Sampled EPSCs (E) and the amplitude of EPSCs (F, mean + s.e.m.) induced by 10 stimuli at 100 Hz at WT (6 neurons, 6 mice), Kv.3.3−/− (5 neurons, 5 mice), and Kv3.3G592R (5 neurons, 5 mice) calyceal synapses of P7–10 mice at 22–24°C. F, amplitudes normalized to the first EPSC (Norm. EPSC Amp) plotted versus stimulation (stim.) number; **: p < 0.01, 2–10th EPSCs in WT are larger than that in Kv.3.3−/− or Kv3.3G592R synapses (t test).