Figure 4.

Kv3.3 KO or Kv3.3_G592R KI inhibits endocytosis at hippocampal synapses

(A-B) F_SypH (mean + s.e.m., A) and Rate_decay (mean + s.e.m., B) induced by 1 AP in WT, Kv.3.3^−/− or Kv3.3_G592R boutons at 34–37°C. F_SypH is normalized to baseline; s.e.m. (A) is plotted every 4 s. The experimental number is written inside the bar (B, applies to panel A-B); each experiment contained 20–30 boutons; 1–3 experiments were taken from 1 culture; each culture was from 3–5 mice. Rate_decay was calculated from F_SypH normalized to the baseline. *: p < 0.05; **: p < 0.01; t test (compared to WT).

(C-F) Similar to A-B, respectively, except that the stimulus was 40 APs (C-D) or 200 APs (E-F) at 20 Hz.

(G-H) Similar to E-F (200 APs), respectively, except from WT, or Kv.3.3^−/− cultures overexpressed with WT Kv3.3 (KO + WT) or a mutant (W496F) Kv3.3 with no ionic conductance (KO + Mutant).

(I-J) Similar to E-F, respectively, except from WT cultures, WT cultures with 20 μM latrunculin A in the bath (WT + Lat A), or Kv.3.3^−/− cultures with Lat A in the bath (KO + Lat A). N.S.: not significant (p > 0.05, t test).

See also Figures S1–S4.