KEY RESOURCES TABLE
| REAGENT or RESOURCE | SOURCE | IDENTIFIER |
|---|---|---|
| 3,3-Diaminobenzidine tetrahydrochloride (DAB) | EMS | 13080 |
| 3,4-Diaminopyridine (3.4-DAP) | Sigma | D7148 |
| 6-Cyano-7-nitroquinoxaline-2, 3-dione (CNQX) | Tocris | 0190 |
| Absolute Ethanol | Decon Labs | 2716 |
| B27 supplement, serum free | Gibco | 17504044 |
| Bicuculline | Sigma | 14340 |
| BioMix Red 2X | Thermo Fisher Scientific | BIO25006 |
| Biotinylated goat anti rabbit antibody | Vector Labs | BA-1000 |
| Bovine serum albumin | Sigma | A2153 |
| Bovine serum albumin | American Analytical | AB01088 |
| Collagenase P | Sigma | 11249002001 |
| Cytosine β-D-arabinofuranoside | Sigma | C1768 |
| D(−)-2-Amino-5-phosphonovaleric acid | Tocris | 3693 |
| DMEM medium | Thermo Fisher Scientific | 11885–092 |
| DNase | Worthington | LK003172-D2 |
| Durcupan ACM Epoxy Resin | EMS | 14040 |
| Embed-812 | EMS | 13940 |
| Fetal Bovine Serum | Thermo Fisher Scientific | 10082–147 |
| Formvar | EMS | 15810 |
| German glass coverslips with mouse Laminin coating over PDL layer | Neuvitro | GG-25-Laminin |
| GlutaMAX-1 | Gibco | 35050–061 |
| HBSS (with Ca and Mg) | Thermo Fisher Scientific | 14025–134 |
| HBSS (without Ca and Mg) | Thermo Fisher Scientific | 14175–103 |
| HEPES | Thermo Fisher Scientific | 15630080 |
| HT supplement | Thermo Fisher Scientific | 11067030 |
| IMDM medium | Thermo Fisher Scientific | 12440053 |
| Insulin | Sigma | I5500 |
| Latrunculin A | Tocris | 3973 |
| Lipofectamine 2000 | Thermo Fisher Scientific | 11668019 |
| Lipofectamine LTX with PLUS | Thermo Fisher Scientific | 15338–100 |
| Neurobasal-A | Thermo Fisher Scientific | 10888–022 |
| Normal goat serum | Vector Laboratories | S-1000–20 |
| NucBlue Live ReadyProbes | Thermo Fisher Scientific | R37605 |
| Opti-MEM | Thermo Fisher Scientific | 31985070 |
| Osmium Tetroxide | EMS | 19140 |
| Ovomucoid inhibitor | Worthington | LK003182 |
| Papain | Worthington | LK003178 |
| Penicillin-Streptomycin solution | Thermo Fisher Scientific | 15140–122 |
| Phosphate buffered saline (PBS) | Gibco | 10010023 |
| Pierce 16% formaldehyde, methanol free | Thermo Fisher Scientific | 28908 |
| Propylene Oxide | EMS | 20401 |
| Rabbit Kv3.3 antibody | Alomone Labs | APC-102 |
| Single slot grids | EMS | M2010-CR |
| Tetraethylammonium chloride (TEA-Cl) | Sigma | T2265 |
| Tetrodotoxin (TTX) | Tocris | 1078 |
| Triton X-100 | Sigma | T8787 |
| Trypsin inhibitor | Sigma | T9253 |
| Uranyl Acetate | EMS | 22400 |
| Basic Primary Neurons Nucleofector Kit | Lonza | VVPI-1003 |
| Elite ABC-HRP kit | Vector Labs | PK-6100 |
| QuickExtract DNA Extraction Solution | Lucigen | QE09050 |
| QuikChange II XL Site-Directed Mutagenesis Kid | Agilent | 200521 |
| CHO cell | Kaczmarek lab | CHO/DHFR (−) |
| G592R Kv3.3 knock-in mice | Kaczmarek lab | N/A |
| Kv3.1 KO mice | Kaczmarek lab | N/A |
| Kv3.3 KO mice | Kaczmarek lab | N/A |
| B6 line mice | Kaczmarek lab | N/A |
| Kv3.3 WT | Kaczmarek lab | N/A |
| pH-sensitive pHluorin 2X (SypH) | Yong-Ling Zhu lab | N/A |
| rKv3.3/pcDNA3 | Kaczmarek lab | N/A |
| W496F Kv3.3 mutant | Kaczmarek lab | N/A |
| Clampfit 9 | Molecular Devices | https://mdc.custhelp.com/app/answers/detail/a_id/18826/~/axon%E2%84%A2-pclamp%E2%AE-9-electrophysiology-data-acquisition-%26-analysis-software-download |
| Huygens Professional | Scientific Volume Imaging | https://svi.nl/Huygens-Professional |
| Igor Pro | WaveMetrics | http://www.wavemetrics.com/ |
| Image J | NIH | https://imagej.nih.gov/ij/ |
| LAS X | Leica | https://www.leicabiosystems.com/ |
| Matlab | MathWorks | https://www.mathworks.com/products/matlab.html |
| NIS-Elements AR 4.1 (Nikon A1 confocal microscope) | Nikon | https://www.microscope.healthcare.nikon.com/products/software/nis-elements/nis-elements-advanced-research |
| Python v3.5.2 | Python Core Team | https://www.python.org |
| Pulse v8.67 (EPC-9 amplifier) | HEKA Elektronik | https://www.heka.com |