Figure 2.
Fluorescence spectra from modified assay without an extrinsic acceptor to identify the acceptor for phospholipid transfer activity in tears.
Fluorescent labeled micelles 6 μl, were incubated for 30 minutes at 37 degrees. Samples from various tear components and control solutions, were made from 40 μl of tears. Fluorescence spectra, λex= 465 nm were taken at after precipitation and centrifugal removal of the remaining micelles. The solutions included whole tears (red), tears without tear lipocalin (orange), purified tear lipocalin (blue) and high density lipoprotein (negative control).