Table 1. Ellipsometry of Tear Components.

Tear components were added in concentrations to approximate the final concentration of the major component in tears. Final concentration of tear lipocalin was 74μM. HMWF (high molecular weight fraction from tears), was added to approximate the final concentration of lactoferrin in tears 19 μM. The range of increased thickness is reported from a minimum 3 repeat samples in which average thicknesses were calculated from all pixels of a scan, with minimum of at least 3 separate regions for each sample.

Sample	Mean increase in thickness (range in nm)
Tear buffer	0.3-0.4
Successive addition of HMWF to tear buffer	0.9-1.0
Successive addition of tear lipocalin to tear buffer #1	1.4-1.7
Successive addition of HMWF to tear lipocalin/tear buffer	0.8-.9
Successive addition DPPC added to above	4.0-4.9