KEY RESOURCES TABLE.
| REAGENT or RESOURCE | SOURCE | IDENTIFIER |
|---|---|---|
| Antibodies | ||
| anti-β-actin | Santa Cruz Biotechnology | Cat# sc-47778; RRID: AB_626632 |
| anti-SOX2 (For IB) | Santa Cruz Biotechnology | Cat# sc-365823; RRID AB_10842165 |
| anti-PRMT6 (For IF) | Santa Cruz Biotechnology | Cat# sc-271744; RRID:AB_10715087 |
| anti-PRMT1 | Santa Cruz Biotechnology | Cat# sc-59648; RRID: AB_785301 |
| anti-CK2α | Santa Cruz Biotechnology | Cat# sc-12738, RRID:AB_2276843 |
| anti-Ran | Santa Cruz Biotechnology | Cat# sc-271376; RRID:AB_10610890 |
| anti-p21 | Santa Cruz Biotechnology | Cat# sc-6246; RRID:AB_628073 |
| anti-PRMT6 (For IB and IP) | Cell Signaling Technology | Cat# 14641; RRID:AB_2798552 |
| anti-S139-H2A.X | Cell Signaling Technology | Cat# 9718S; RRID:AB_2118009 |
| anti-H3 | Cell Signaling Technology | Cat# 9715; RRID:AB_331563 |
| anti-pS10H3 | Cell Signaling Technology | Cat# 9701; RRID:AB_331535) |
| anti-SOX2 (For IF) | Cell Signaling Technology | Cat# 3579S; RRID:AB_2195767 |
| anti-OLIG2 | R&D Systems | Cat# AF2418-SP; |
| anti-c-Myc | Cell Signaling Technology | Cat# 18583; |
| anti-Ran (For IF) | Novus Biologicals | Cat# NBP2-61832 |
| anti-Stat3 | Cell Signaling Technology | Cat#12640; RRID:AB_2629499 |
| anti-cleaved Caspase-3 (Asp175) | Cell Signaling Technology | Cat# 9661S; RRID:AB_2341188 |
| anti-HaloTag | Promega | Cat# G9211; RRID:AB_2688011 |
| anti-H4R3me2a | Active Motif | Cat# 39705; RRID:AB_2793313 |
| anti-HA.11 | BioLegend | Cat# 901515; RRID:AB_2565334 |
| anti-H3R2me2 | GeneTex | Cat# GTX54134; RRID:AB_2752252 |
| Anti-p-RCC1 (Ser11) | Cell Signaling Technology | Cat# 5500; RRID:AB_2797613 |
| anti-PRMT6 (for IHC and IF) | Abcam | Cat# ab72205; RRID:AB_1270032 |
| anti-RCC1 | Abmart | Cat# ab36760; RRID:AB_2179587 |
| anti-Flag | Sigma-Aldrich | Cat# F3165;RRID:AB_259529 Cat# F7425; RRID:AB_439687 |
| anti-α-Tubulin | Sigma-Aldrich | Cat# T9026; RRID:AB_477593 |
| anti-p-Ser/Thr | BD Biosciences | Cat# 612549; RRID:AB_399844 |
| anti-RCC1 R214me2 | Genemed Synthesis, | this manuscript |
| anti-p-S11-PRMT6 | ABclonal Science | this manuscript |
| anti-p-T21-PRMT6 | ABclonal Science | this manuscript |
| Peroxidase-IgG Fraction Monoclonal Mouse Anti-Rabbit IgG, Light Chain Specific | Jackson ImmunoResearch Laboratories | Cat# 211-032-171; RRID:AB_2339149 |
| anti-Ki-67 | EMD Millipore | Cat# AB9260; RRID:AB_2142366 |
| anti-αDMA | EMD Millipore | Cat# 07-414; RRID:AB_310596 |
| Alexa Fluor 488 | Thermo Fisher Scientific | Cat# A-11008; RRID:AB_143165 |
| Alexa Fluor 594 | Thermo Fisher Scientific | Cat# R37117; RRID:AB_2556545 |
| Horseradish Peroxidase Streptavidin antibody | Vector Laboratories | Cat# SA-5004; RRID:AB_2336509 |
| Anti-Mouse IgG (H+L), rat adsorbed, made in horse antibody | Vector Laboratories | Cat# BA-2001; RRID:AB_2336180 |
| Biotinylated Goat Anti-Rabbit IgG antibody | Vector Laboratories | Cat# BA-1000; RRID:AB_2313606 |
| anti-Rabbit Immunoglobulins | DAKO | Cat# P0217 |
| anti-Mouse Immunoglobulins | DAKO | Cat# P0260 |
| anti-Goat Immunoglobulins | DAKO | Cat# P0449 |
| Biological Samples | ||
| Paraffin-emended glioma tissues | Saitama Medical University | Saitama, Japan |
| snap-frozen glioma tissue | Northwestern University | Chicago, USA |
| normal brain tissues | NeuroBioBank, NIH | Bethesda, MD, USA |
| Chemicals, Peptides, and Recombinant Proteins | ||
| aDMA of R214me2 RCC1 peptide | Genemed Synthesis Inc. | this manuscript |
| Unmodified R214 RCC1 peptide | Genemed Synthesis Inc. | this manuscript |
| p-T21 PRMT6 peptide | ABclonal Science Inc | this manuscript |
| Unmodified T21 PRMT6 peptide | ABclonal Science Inc | this manuscript |
| p-S11 PRMT6 peptide | ABclonal Science Inc | this manuscript |
| Unmodified S11 PRMT6 peptide | ABclonal Science Inc | this manuscript |
| SB 216763 | Sigma-Aldrich | Cat# S3442 |
| D4476 | Sigma-Aldrich | Cat# 218696 |
| KN-93 | Sigma-Aldrich | Cat# K1385 |
| H-89 | Sigma-Aldrich | Cat# B1427 |
| RO-3306 | Sigma-Aldrich | Cat# SML0569 |
| SB 202190 | Sigma-Aldrich | Cat# S7067 |
| Roscovitine | Sigma-Aldrich | Cat# R7772 |
| Cycloheximide | Sigma-Aldrich | Cat# C7698 |
| SAM | Sigma-Aldrich | Cat# A4377 |
| Thymidine | Sigma-Aldrich | Cat# T9250 |
| Nocodazole | Sigma-Aldrich | Cat# M1404 |
| CX-4945 | AdooQ BioScience | Cat# A11060 |
| EPZ020411 | AdooQ BioScience | Cat# A16377 |
| Vectashield mounting medium with DAPI | Vector Laboratories | Cat# H-1200 |
| MG-132 | Selleckchem | Cat# S2619 |
| Lipofectamine 2000 | Thermo Fisher Scientific | Cat# 11668019 |
| Kinase buffer | Cell Signaling Technology | Cat# 9802 |
| ATP | Cell Signaling Technology | Cat# 9804 |
| D-Luciferin | Gold Bio | Cat# PP82 |
| AquaBlock | EastCoast Bio | Cat# 2144 |
| Protease Inhibitor Cocktail | Sigma-Aldrich | Cat# 11836153001 |
| Phosphatase inhibitor | Sigma-Aldrich | Cat# 4906837001 |
| Protein G Agarose | Sigma-Aldrich | Cat# 16-266 |
| 2% paraformaldehyde/2.5% glutaraldehyde | Polysciences | Cat# 22872 |
| IP Lysis Buffer | Thermo Fisher Scientific | Cat# 87788 |
| O.C.T Compound | Thermo Fisher Scientific | Cat# 23-730-571 |
| Critical Commercial Assays | ||
| QuikChange mutagenesis kit | Agilent Technologies | Cat# 200524 |
| CellTiter Glo 2.0 | Promega | Cat# 200515-5 |
| NE-PER™ Nuclear and Cytoplasmic Extraction Reagents | Thermo Scientific | Cat# 78833 |
| QIAquick Gel Extraction kit | Qiagen | Cat#59104 |
| SYBR Select Master Mix | Life Technologies | Cat#4472913 |
| RAN Activation Assay kit | Cell Biolabs | Cat# STA-409 |
| Q5® Site-Directed Mutagenesis Kit | New England Biolabs | Cat# E0554S |
| Chromatin Extraction Kit | Abcam | Cat# ab117152 |
| Deposited Data | ||
| RNA Seq data of Northwestern glioma cohort | NCBI GEO | GSE147352 |
| Original data that associates with this study | Mendeley Data | https://data.mendeley.com/datasets/942x54gf5t/draft?a=d807130b-bb51-4a30-b84b-bb72335f682b |
| Experimental Models: Cell Lines | ||
| U87 | ATCC | Cat# HTB-14 |
| T98G | ATCC | Cat# CRL-1690 |
| LN443 | (Ishii et al., 1999) | N/A |
| LN444 | (Ishii et al., 1999) | N/A |
| GSC19 | (Mao et al., 2013) | N/A |
| GSC84 | (Mao et al., 2013) | N/A |
| GSC157 | (Mao et al., 2013) | N/A |
| GSC83 | (Mao et al., 2013) | N/A |
| HEK293T | ATCC | Cat# CRL-3216 |
| GSC46 | (Srikanth et al., 2013) | N/A |
| GSC23 | (Bhat et al., 2013) | N/A |
| GSC576 | (Rohle et al., 2013) | N/A |
| NPC or NHNP | LONZA | Cat# PT-2599 |
| NHA | LONZA | Cat# CC-2565 |
| Experimental Models: Organisms/Strains | ||
| Athymic (Ncr nu/nu) mice | Taconic Farms | NCRNU-F and NCRNU-M |
| Oligonucleotides | ||
| PRMT6-KO construction primers on lentiCRISPv2GFP or PX458 | Table S5 | N/A |
| PRMT6 qPCR primers | Table S5 | N/A |
| SOX2 qPCR primers | Table S5 | N/A |
| OLIG2 qPCR primers | Table S5 | N/A |
| TUBB3 qPCR primers | Table S5 | N/A |
| ACTB qPCR primers | Table S5 | N/A |
| Recombinant DNA | ||
| TRC shRNA Control | Dharmacon | Cat#RHS6848 |
| GIPZ shRNA Control | Dharmacon | Cat#RHS4346 |
| PRMT6 shRNA #3 | Dharmacon | V2LHS_155923 |
| PRMT6 shRNA #4 | Dharmacon | TRCN0000034685 |
| RCC1 shRNA #2 | Dharmacon | TRCN0000152440 |
| RCC1 shRNA #3 | Dharmacon | TRCN0000157236 |
| CK2α shRNA #1 | Dharmacon | TRCN0000000606 |
| CK2α shRNA #2 | (Ji et al., 2009) | N/A |
| CK2α shRNA#3 | (Ji et al., 2009) | N/A |
| pcDNA3 CK2α and mutants | this manuscript | N/A |
| CD530-PRMT6-WT | this manuscript | N/A |
| CD530-PRMT6-KLA | this manuscript | N/A |
| CD530-PRMT6-S11A | this manuscript | N/A |
| CD530-PRMT6-S11D | this manuscript | N/A |
| CD530-PRMT6-T21A | this manuscript | N/A |
| CD530-PRMT6-T21D | this manuscript | N/A |
| CD530-PRMT6-2A | this manuscript | N/A |
| CD530-PRMT6-2D | this manuscript | N/A |
| CD530-PRMT6 domain deleted | this manuscript | N/A |
| pLvx-PRMT6-Myc | this manuscript | N/A |
| pCMV6-myc-WT | this manuscript | N/A |
| CD530-RCC1-WT | this manuscript | N/A |
| CD530-RCC1-R214K | this manuscript | N/A |
| CD530-RCC1-R217K | this manuscript | N/A |
| CD530-PRMT6-S11D | this manuscript | N/A |
| CD530-RCC1 domain deleted | this manuscript | N/A |
| pLVX-EGFP-RCC1 mutants | this manuscript | N/A |
| Halo-PRMT1-8 | (Wang et al., 2014) | N/A |
| pET28a –PRMT6 WT and mutants | this manuscript | N/A |
| pET28a –RCC1 WT | this manuscript | N/A |
| psPAX2 | Addgene | Cat#12260; RRID:Addgene_12260 |
| pCMV-VSV-G | Addgene | Cat#8454; RRID:Addgene_8454 |
| Software and Algorithms | ||
| Firebrowse | N/A | http://firebrowse.org/ |
| Gliovis | N/A | http://gliovis.bioinfo.cnio.es/ |
| gRNA design MIT online tool | N/A | https://zlab.bio/guide-design-resources |
| GraphPad Prism 6.0 | GraphPad | https://www.graphpad.com/ |
| Extreme Limiting Dilution Analysis (ELDA) | (Hu and Smyth, 2009) | http://bioinf.wehi.edu.au/software/elda/ |