Extended Data Figure 4. Flow cytometry gating strategy for the isolation of hematopoietic stem and progenitor cell populations.

(a-d) Representative flow cytometry gates used to identify the hematopoietic stem and progenitor cell populations in the bone marrow (a, b), and T lineage progenitors in the thymus (c, d). The markers used to identify each of the cell populations characterized in this study are listed in Supplementary Table 1. (e) Representative flow cytometry gates showing that more than 50% of IL7Rα⁺Lineage⁻ cells were Lin⁻Sca1^lowc-kit^lowIL7Ra⁺Flt3⁺ CLPs and most of the non-CLP IL7Rα⁺Lineage⁻ (Flt3⁻) cells were CD19⁺, likely to be other early B lineage progenitors (4 mice analyzed in 3 independent experiments). All data represent mean ± SD.