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. Author manuscript; available in PMC: 2021 Aug 25.
Published in final edited form as: Nat Plants. 2021 Feb 25;7(3):342–352. doi: 10.1038/s41477-021-00863-8

Extended Data Fig. 4. Bottom loading EV separation by sucrose gradient centrifugation.

Extended Data Fig. 4

a, Pellets obtained from 100,000 g centrifugations (P100) were used to perform sucrose gradient centrifugation by both top and bottom loading. b, Six fractions were collected from bottom loading plant EV sucrose gradient centrifugation. TET8, AGO1, RH11, RH37, ANN1, ANN2 were detected by western blot. EV enriched (TAS1c-siR483, TAS2-siR453 and miR166) and non-EV-enriched (TAS1c-siR585 and TAS2-siR710) sRNAs were detected by RT-PCR. The experiments were repeated three times independently with similar results. Source data are provided as a Source Data file.