Extended Data Fig. 8. Verification of rh11rh37 and ann1ann2 double mutants.
a, The developmental phenotypes of the rh11rh37 double mutant that both RH11 and RH37 expression was suppressed by artificial miRNA in Col-0. b, The real-time RT-PCR analysis of RH11 and RH37 expression in rh11rh37 mutants. The data are presented as mean ± s.d., n = 3 biologically independent replicates. The error bars indicate the standard deviation (s.d.). c, The developmental phenotypes of the rh11rh37#6 mutant that RH37 expression was suppressed by artificial miRNA in rh11 knockout mutant. d, RT-PCR analysis of RH11 and real-time PCR analysis of RH37 in rh11rh37#6 mutant. The data are presented as mean ± s.d., n = 3 biologically independent replicates. The error bars indicate the standard deviation (s.d.). e, Phenotype of wild-type and ann1ann2 mutant grown for 4 weeks in a growth chamber. f, RT-PCR analysis of the expression levels of ANN1 and ANN2 in wild-type and ann1ann2 double mutant. The statistical analysis was performed using unpaired two-tailed Student’s t-tests. The small open circles represent the individual values. The asterisks indicate significant differences: *P < 0.05, **P < 0.01, ***P < 0.001. The experiments were repeated three times independently with similar results. Source data are provided as a Source Data file.