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. Author manuscript; available in PMC: 2021 Aug 25.
Published in final edited form as: Nat Plants. 2021 Feb 25;7(3):342–352. doi: 10.1038/s41477-021-00863-8

Extended Data Fig. 8. Verification of rh11rh37 and ann1ann2 double mutants.

Extended Data Fig. 8

a, The developmental phenotypes of the rh11rh37 double mutant that both RH11 and RH37 expression was suppressed by artificial miRNA in Col-0. b, The real-time RT-PCR analysis of RH11 and RH37 expression in rh11rh37 mutants. The data are presented as mean ± s.d., n = 3 biologically independent replicates. The error bars indicate the standard deviation (s.d.). c, The developmental phenotypes of the rh11rh37#6 mutant that RH37 expression was suppressed by artificial miRNA in rh11 knockout mutant. d, RT-PCR analysis of RH11 and real-time PCR analysis of RH37 in rh11rh37#6 mutant. The data are presented as mean ± s.d., n = 3 biologically independent replicates. The error bars indicate the standard deviation (s.d.). e, Phenotype of wild-type and ann1ann2 mutant grown for 4 weeks in a growth chamber. f, RT-PCR analysis of the expression levels of ANN1 and ANN2 in wild-type and ann1ann2 double mutant. The statistical analysis was performed using unpaired two-tailed Student’s t-tests. The small open circles represent the individual values. The asterisks indicate significant differences: *P < 0.05, **P < 0.01, ***P < 0.001. The experiments were repeated three times independently with similar results. Source data are provided as a Source Data file.