Skip to main content
. Author manuscript; available in PMC: 2021 Aug 25.
Published in final edited form as: Nat Plants. 2021 Feb 25;7(3):342–352. doi: 10.1038/s41477-021-00863-8

Fig. 3. TET8-positive EVs are an important subclass of EVs for secretion of sRNAs and RBPs.

Fig. 3

a, The structures and the topology of plant tetraspanin TET8. The peptide of the large extracellular loop EC2 domain was used for production of TET8 specific antibody. b, Anti-TET8 antibody can detect TET8 in EVs isolated from wild-type plants but not from tet8 mutants. c, TET8-positive EVs can be pulled-down by TET8-specific antibody linked beads. IgG was used as a negative control. d, EV-associated sRNAs are enriched in EVs isolated from TET8 specific antibody pull-down fractions. AGO2- associated miR393* and AGO4-associated siRNA1003 were used as negative control. e, AGO1, AGO2, AGO4, RH11, RH37, ANN1 and ANN2 were detected in anti-TET8 immuno-isolated EV fractions by western blot. AGO2 and AGO4 serve as negative control. Scale bars, 10 μm. The experiments in Fig. 3b, d-e were repeated three times with similar results. Source data are provided as a Source Data file.