Skip to main content
. Author manuscript; available in PMC: 2021 Aug 25.
Published in final edited form as: Nat Plants. 2021 Feb 25;7(3):342–352. doi: 10.1038/s41477-021-00863-8

Fig. 5. EV secreted RBPs mediate sorting and stabilization of sRNAs into EVs.

Fig. 5

a, b, sRNAs were immunoprecipitated from plant total extraction (a) and EV extraction (b) using antibodies raised against AGO1, AGO2 and GFP to detect EV-enriched and non-EV-enriched sRNA association with AGO1, AGO2, RH11, RH37, ANN1 and ANN2. AGO1 and AGO2 proteins were detected by western blot using anti-AGO1 and anti-AGO2 antibodies, respectively. RH11, RH37, ANN1, and ANN2 were detected by western blot using anti-GFP antibodies. IgG was used as a negative control. The experiments in Fig. 5a and b were repeated three times with similar results. Source data are provided as a Source Data file.