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. 2021 Feb 18;40(11):1974–1987. doi: 10.1038/s41388-021-01682-z

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© The Author(s) 2021, corrected publication 2021

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Fig. 1 — a Gene set enrichment analysis (GSEA) of enrichment of MUELLER_PLURINET in nonsmoking patients vs. smoking patients in GSE53625 dataset. FDR false-discovery rate q value. NES normalized enrichment score. b Representative images of immunofluorescence for CK14 and CK18 in KYSE270 cells (upper panel) and TE1 cells (bottom panel) treated with different concentrations (0.1, 1, and 10 μM) of nicotine for 48 h. c Representative images of spheres formed by KYSE270 and TE1 cells treated with nicotine alone or combined with mecamylamine (left panel). Quantification of spheres formed per 1000 cells (right panel). d Flow cytometry analysis of the ALDH-positive population in TE1 cells treated with nicotine or mecamylamine. e Flow cytometry analysis of the CD44-positive population in TE1 cells treated with nicotine or mecamylamine. Data are shown as the means of three independent experiments or representative data. Error bars indicate SD. **P < 0.01, ***P < 0.001 by Student’s t-test or one-way ANOVA with post hoc intergroup comparisons.