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. 2021 Feb 18;40(11):1974–1987. doi: 10.1038/s41388-021-01682-z

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© The Author(s) 2021, corrected publication 2021

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Fig. 2 — a The mRNA levels of the 12 nicotinic acetylcholine receptors derived from an ESCC dataset in GEO (GSE53625), which includes 179 ESCC (tumor) and their paired adjacent normal tissues (nontumor). The red and gray lines connect the nontumor and tumor values in patients with high and low expression, respectively. GSEA plots of enrichment of MUELLER_PLURINET in high expression versus low expression of CHRNA1 (b), CHRNA5 (c), CHRNA6 (d), CHRNA7 (e), CHRNA9 (f), CHRNB1 (g), CHRNB3 (h), CHRNB4 (i) in the GSE53625 dataset. FDR false-discovery rate q value. NES normalized enrichment score. j The mRNA levels of CHRNA5 in KYSE270 and TE1 cells treated with nicotine or vehicle. k Representative images of semi-quantitative PCR analysis of CHRNA5 and CHRNA7 in KYSE270 and TE1 cells treated with or without nicotine. GAPDH was used as an internal loading control. l Immunoblotting analyses of CHRNA7 expression in ESCC cell lines and immortalized normal esophageal epithelial cell lines. Data are shown as the means of three independent experiments or representative data. Error bars indicate SD. *P < 0.05, **P < 0.01, ***P < 0.001 by paired t-test.