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. 2021 Feb 18;40(11):1974–1987. doi: 10.1038/s41388-021-01682-z

Fig. 4. The effect of CHRNA7 on ESCC CIC properties in vivo.

Fig. 4

a RT-qPCR (top panel) and immunoblot (bottom panel) analysis of CHRNA7 in KYSE510 cells transfected with CHRNA7 overexpression plasmid or empty vector. GAPDH was used as an internal control. b The table showing that the P value of each case in which the nude mice (n = 8 per group) were injected with KYSE510 cells transfected with CHRNA7 overexpression plasmid or empty vector. c Tumors derived from nude mice injected KYSE510 cells stably transfected with CHRNA7-expressing plasmid or empty vector at the end of the experiments. d Immunoblot of CHRNA7-JAK2-STAT3-SOX2 in representative tumors derived from nude mice injected KYSE510 cells stably transfected with CHRNA7-expressing plasmid or empty vector. e IHC of CHRNA7-JAK2-STAT3-SOX2 in representative tumors derived from KYSE510 stably transfected with CHRNA7-expressing plasmid or empty vector. GAPDH was used as an internal control. Data are shown as the means of three independent experiments or representative data. Error bars indicate SD. ***P < 0.01 by Student’s t-test.