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. 2021 Feb 18;40(11):1957–1973. doi: 10.1038/s41388-021-01685-w

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 4 — a–c Effects of stable PDGFC knockdown of CAFs on Transwell migration and invasion assays of T1 cells. Experimental design for the Transwell assays (a) and representative images of the migrated cells (b). The quantitative data (c) were generated with migrated cells that were counted by ImageJ software. p values were represented by ANOVA analysis. **p < 0.01, ***p < 0.001. d Wound healing assay in the indicated conditions. Data represents average of % covered gap size. p values were represented by ANOVA analysis. **p < 0.01, ***p < 0.001. e, f Spleen-to-liver metastasis model showing the effect of PDGFC secreted from CAFs. The mice were injected with mCherry-labeled T1, T1 + CAFscr, T1 + CAFshPDGFC #1, and T1 + CAFshPDGFC #2. IVIS images of metastatic liver (e) and quantification (f). Liver metastasis burden was quantified by total photon flux (p/s) on day 21 after the cell injection. p values were represented by ANOVA analysis. ***p < 0.001. Representative hematoxylin and eosin (H&E) images (g) and IHC images (h) stained for KIT in the tumor section collected from metastatic liver in Fig. E. Scale bars, 100 µm.