Table 3.
AUC (pmol×min/µL), incorporation coefficient (K*) (µL/(mg×min), incorporation rate, Jin ((pmol/(mg×min)), turnover, F (min−1), half life, t1/2 (min) in visceral adipose, liver, and heart in IV and gavage rats.
| IV (UE) | Gavage (ES) | ||
|---|---|---|---|
| Plasma d4–13-HODE AUC | 16.4 ± 19.3 | 2.1 ± 1.71 | |
| Visceral adipose | k* | 0.047 ± 0.041 | 0.88 ± 1.16 |
| Liver | k* (×10−4) | 3.6 ± 3.8 | 22.9 ± 12.2ϯ |
| Heart | k* (×10−4) | 20.3 ± 21.1 | 42.4 ± 23.0 |
| Visceral adipose | Jin (×10−4) | 22.2 ± 17.1 | 846.2 ± 1250.0 |
| Liver | Jin (×10−4) | 0.18 ± 0.15 | 1.9 ± 1.3ϯ |
| Heart | Jin (×10−4) | 1.0 ± 1.0 | 3.2 ± 1.7 |
| Visceral adipose | F (×10−4) | 1.6 ± 1.5 | 65.1 ± 72.3ϯ |
| Liver | F (×10−4) | 0.15 ± 0.075 | 1.7 ± 1.1 ϯϯ |
| Heart | F (×10−4) | 0.7 ± 0.6 | 2.2 ± 1.5 |
| Visceral adipose | 𝑡1/2 (×104) | 0.9 ± 0.9 (6.2 days) | 0.05 ± 0.07 (0.34 day)ϯ |
| Liver | 𝑡1/2 (×104) | 5.5 ± 2.3 (38.3 days) | 0.5 ± 0.3 (3.6 days)ϯϯ |
| Heart | 𝑡1/2 (×104) | 2.1 ± 2.3 (14.7 days) | 0.4 ± 0.3 (3.1 days) |
Data are mean ± SD of n=3 per group.
ϯ
Indicates significant differences in log-transformed values between IV and gavage groups, by unpaired t-test applied to log-transformed values.
1
Esterified d4–13-HODE AUC determined by subtracting free from total d4–13-HODE AUC in each individual rat that received the tracer via gavage.