Fig. 1.

Aurora kinase overexpression induces resistance to T-cell-mediated cytotoxicity. a A high-throughput ORF screen showed that AURKB overexpression enhanced resistance to T-cell-mediated cytotoxicity. The cytotoxicity induced by expression of an ORF alone (x axis) versus cytotoxicity induced by the combination of an ORF and autologous T cells (y axis) in Mel2549 cells is shown. Each circle represents one ORF, while the color intensity represents the comboscore (a comboscore of 1, representing no additional effect of T-cell treatment, is shown in gray). The AURKB ORF is indicated by an arrow and solid circle (comboscore = 0.45). Overexpression of Aurora kinases A and B in Mel2338 (b) and Mel2549 (c) was confirmed to decrease comboscores compared with the GFP control. Comboscores ( +) were superimposed on the cleaved caspase-3 percentages induced by ORF alone (Δ), TIL alone (o), and ORF plus TIL (◊). BCL2L1 overexpression is shown as a positive control for suppression of T-cell cytotoxicity