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. 2021 Jan 22;20(2):733–750. doi: 10.1007/s10237-020-01413-5

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© The Author(s) 2021

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 2 — LAL surgery. a Schematic representation of LAL steps. b Representative pictures of LAL steps. Step 1 Opening chorionic and allantoic membranes and exposing the animal. Step 2 Flipping the animal vertically to expose its left side shown in Step 3. Step 4 Opening the pericardium and exposing the left atrium. Step 5 Placing the pre-prepared surgical knot on the top of the atrium. Step 6 Tightening the knot around the atrium. Step 7 Cutting the extra ends of the suture and finally flipping back the animal to its original orientation