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. 2021 Jan 22;20(2):733–750. doi: 10.1007/s10237-020-01413-5

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© The Author(s) 2021

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Fig. 3 — Echocardiography imaging for an HH21 embryo. B-mode images and Doppler velocity measurements for AV canal. In B-mode images, AVC is atrioventricular cushion. Arrow shows the blood flow direction. Edges of atrial and ventricular myocardium are highlighted in black. Edges of AVC are highlighted in yellow. Doppler blood velocity profile shows two peaks which correspond to initial ventricular relaxation and atrial contraction/ventricular filling phases