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. 2021 Jan 22;20(2):733–750. doi: 10.1007/s10237-020-01413-5

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© The Author(s) 2021

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 4 — Micro-CT cast creation and 3D volume generation. a CT-dense contrast agent is perfused through cardiovascular system for cast creation. Micro-CT scanning enables generation of 3D heart volumes. b Unseptated 3.5-day (HH21) heart. c Septated 7-day (HH30) heart. d Orifice sizes for right and left AV canals in c can be seen through a section inside atria. RAV right atrioventricular canal, LAV left atrioventricular canal, R right, L left, RA right atria, LA left atria, RV right ventricle, LV left ventricle