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. 2021 Jan 22;20(2):733–750. doi: 10.1007/s10237-020-01413-5

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© The Author(s) 2021

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 9 — LAL causes anatomical changes on ED7 chick hearts 72-h post-surgery. ED7 control and LAL hearts are oriented and H&E processed. Different parameters are measured using ImageJ. a Demonstration of the quantified parameters shown in b–g. h Demonstration of the parameters quantified in i–l. LA left atrium, LV left ventricle, RA right atrium, RV right ventricle. Data is presented as mean (n = 3), error bars indicate SEM, and p < 0.05 indicates statistical significance as determined by Student’s t test (*p < 0.05)