Table 3.

Result of delayed entry on organism TTD in FA PLUS, PF PLUS, and FN PLUS bottles on VIRTUO

Organism (strain #) actual CFU/bottle	Delayed entry time (h) and holding temperature (°C)
	No delay				24 h (20–25 °C)				36 h (20–25 °C)
	Avg. TTD (h)	TTD range (h)	# (+)/n	Detectionrate (%)	Avg. TTD (h)	TTD range (h)	# (+)/n	Detectionrate (%)	Avg.TTD (h)	TTDrange (h)	# (+)/n	Detectionrate (%)
Acinetobacter baumannii (19606) 11 CFU, (102460) 9 CFU, (105298) 5 CFU	12.0	10.1–14.7	81/81	100	6.0	3.8–10.0	81/81	100	3.8	2.7–7.7	79/81	97.5
Bacteroides fragilis (25285) 62 CFU	32.7	27.5–40.5	18/18	100	24.5	20.2–30.3	17/17	100	20.2	15.4–25.8	18/18	100
Candida albicans (14053) 26 CFU	26.9	23.0–30.5	26/26	100	12.4	10.0–14.0	27/27	100	5.8	2.8–9.8	27/27	100
Clostridium perfringens (13124) 47 CFU	10.3	9.1–13.0	18/18	100	6.8	5.5–9.6	18/18	100	3.1	2.8–3.7	18/18	100
Enterococcus faecium (35667) 12 CFU	10.6	9.2–15.2	45/45	100	4.2	3.4–5.5	45/45	100	2.4	1.5–3.2	45/45	100
Escherichia coli (25922) 16 CFU	9.7	9.0–10.9	45/45	100	4.1	3.5–5.5	45/45	100	2.3	1.7–2.9	45/45	100
Haemophilus influenzae (10211) 26 CFU	12.5	11.7–13.3	27/27	100	10.4	9.2–12.0	27/27	100	9.3	8.5–10.0	27/27	100
Neisseria meningitidis (13090) 40, 50 CFU	18.8	15.7–26.2	20/20	100	18.4	16.1–22.6	18/18	100	21.3	16.6–29.1	18/18	100
Pseudomonas aeruginosa (9027) 22 CFU, (27853) 30 CFU	14.3	11.2–16.3	54/54	100	7.2	6.1–9.7	54/54	100	4.3	3.2–6.0	54/54	100
Staphylococcus aureus (25923) 25 CFU	13.2	10.0–21.2	45/45	100	9.1	5.5–16.7	45/45	100	7.0	3.8–14.0	45/45	100
Streptococcus pneumoniae (6305) 11 CFU,(49619) 84 CFU, (10076) 9 CFU	11.3	8.5–14.7	81/81	100	9.2	7.7–14.1	81/81	100	8.8	6.4–13.0	81/81	100