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. 2020 Oct 2;70(4):893–907. doi: 10.1007/s00262-020-02734-1

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© The Author(s) 2020

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Fig. 5 — Immunohistochemical analysis of resected tumor tissues of patients with a combination therapy of thermoablation and surgical resection (n = 18). a Heatmap showing cell densities of CD3, CD8, CD4, CD20, CD38 and FoxP3 expressing cells in the center of the tumor (CT) and the invasive margin (IM) normalized to the median cell density of each marker for each region dividing them into either high (> median) or low (< median) infiltrated areas. Below, CD3/CD8 Immunoscore (IS), CD8/CD20 TB-Score (TB), overall (OS) and disease-free survival (DFS) are depicted for each patient. b Kaplan Meyer analysis of DFS depending on either high CD3 infiltrates (left) in CT and IM (CD3 high) or low CD3 infiltrates and heterogenous CD3 infiltrates in CT and IM (CD3 low + het) or rather depending on high IS (IS 3–4) or low IS (IS 0–2) (right)