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. 2021 Mar 19;11:6433. doi: 10.1038/s41598-021-85750-0

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© The Author(s) 2021

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Overall analysis workflow and two-dimensional projection of functional profiles. (A). Each microbiome sequencing sample is annotated with PRROMenade, utilizing labeled reference data from the IBM Functional Genomic Platform. The resulting functional profiles are visualized and compared in downstream analyses. (B). Multidimensional scaling of the functional profiles using the Spearman distance. Each sample is represented by a marker colored by cohort and labeled by the sample number within that cohort.