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. 2021 Mar 19;11:6414. doi: 10.1038/s41598-021-85585-9

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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(A) shows weekly average SPA in the first, fifth and tenth weeks for the control (C) and trained (T) groups kept in small cage (SC) and large cage (LC). A time-of-day variation of SPA is exhibited in (B) demonstrating the robustness and sensibility of gravimetric method. This analysis considers all SPA records obtained in first, fifth and tenth weeks (N = 21 for each hour-time interval from 12:00 to 6:00 h). Animal handling (between 6:00 and 12:00 h) includes HIIT, cage cleaning and weighting control. SPA was measured on a per cage basis (10 mice per cage). Statistical significance symbols: a—different from SC-C, b—different from SC-T and c—different from LC-C within the same experimental week (P < 0.05).