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. 2021 Mar 19;12:1746. doi: 10.1038/s41467-021-21932-8

Fig. 1. Mcm7 C-WHD is required for MCM-Cdt1 recruitment.

Fig. 1

a The recruitment of the individual Mcm subunits (M2, 4, 5, 6 and 7) by the OC complex (ORC and Cdc6 bound to a PCR product containing ARS305 and 1049 bp long) was followed using recruitment conditions (ATPγS and low salt washes), under the presence (+) or absence (−) of Mcm3. 20% of each Mcm subunit tested is shown in the input lane. Relative position in the Mcm2-7 ring, of Mcm3 and the subunit tested, are highlighted in black at the top of the figure. Note that the Orc1 subunit and Mcm3 run in similar positions in the gel. b Neighbouring dimers of Mcm3 are tested for recruitment, as in a. c Recruitment of the trimer, Mcm5/3/7, using either Mcm5wt (wt) or increasing amounts of an Mcm5 containing a deletion of the C terminus winged helix domain (C-WHD, M5∆C). Loading factors are labelled in grey and Mcm2-7 in black. d Recruitment of the heptameric MCM-Cdt1 complex containing either Mcm5 wt (lane 1) or with increasing amounts of M5∆C (lanes 2–4). e Recruitment of the trimer Mcm5/3/7, with either Mcm7 wt (lane 1) or increasing amounts of an Mcm7 protein that lacks its C-WHD (M7∆C1, lanes 2–4). f Recruitment of the MCM-Cdt1 with either Mcm7 wt (lane 1) or with increasing amounts of M7∆C1 (lanes 2–4). See also Supplementary Fig. 2c–e.