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. 2021 Mar 19;12:1758. doi: 10.1038/s41467-021-22021-6

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© The Author(s) 2021

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Fig. 3 — a DAPI staining and immunostaining for SYCP1 (red), SYCP3 (green), and VASA (cyan) in SSC cultures with indicated treatment. Scale bars, 10 µm. b Representative chromosome spreads stained by SYCP1 (red) and SYCP3 (green) from SSC cultures following indicated treatment are shown. c Percentages of germ cells at the indicated stages in culture on each day following indicated treatment (n = 200 cells). Scale bar, 5 µm. d DAPI staining and immunostaining for SYCP1 (red), SYCP3 (green), and VASA (cyan) in SSC cultures with indicated treatment for 2 days, followed by treatment of meiotic progression medium for 4 days. Scale bars, 10 µm. e Percentages of germ cells at the indicated stages in culture on each day following indicated treatment (n = 200 cells).