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. 2021 Mar 19;12:1758. doi: 10.1038/s41467-021-22021-6

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 5 — a DAPI staining and immunostaining for SYCP1 (red), SYCP3 (green), and VASA (cyan) in Stra8^+/+ and Stra8^−/− SSC cultures with indicated treatment. Scale bars, 10 µm. b Representative chromosome spreads stained by SYCP1 (red) and SYCP3 (green) from Stra8^+/+ and Stra8^−/− SSC cultures. Percentages of germ cells at the indicated meiotic stages are shown below (n = 50 cells). c DAPI staining and immunostaining for SYCP1 (red), SYCP3 (green), and VASA (cyan) in Spo11^+/+ and Spo11^−/− SSC cultures with indicated treatment. Scale bars, 10 µm. d Representative chromosome spreads stained by SYCP1 (red) and SYCP3 (green) from Spo11^+/+ and Spo11^−/− SSC cultures. Percentages of germ cells at the indicated meiotic stages are shown below (n = 50 cells).