Fig. 2. A subpopulation of melanoma cells can rapidly and reversibly escape BRAF inhibition.
a Schematic of CDK2 sensor26–29. Adapted from refs. 26,27,29, with permission from Elsevier. b Representative single-cell traces of CDK2 activity in an untreated A375 cell (upper panel), and a 1-µM dabrafenib-treated escapee and non-escapee (lower panel). c Heatmap of single-cell CDK2 activity traces in 1-µM dabrafenib-treated A375 and WM278 cells. Each row represents the CDK2 activity in a single-cell over time according to the colormap. Apoptotic cells (Supplementary Fig. 1c) are not included in the heatmap. The percentages mark the proportion of cells with each behaviour. Arrow and black line mark the time of drug addition. d Schematic diagram of the drug holiday experimental setup described in the text. e Cell count over time as measured by time-lapse microscopy after a 24-h drug holiday; each condition was measured in triplicate and plotted individually.