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. 2021 Mar 19;12:1736. doi: 10.1038/s41467-021-21993-9

Fig. 6. PRAK expression is elevated in metastatic lesions of human cancer.

Fig. 6

a Prak mRNA expression was quantified by real-time RT-PCR in 60 lung carcinoma samples with or without recurrence over a 5-year follow-up (30 for each group). Each symbol represents an individual sample. Bars are the mean and error bars represent s.e. **p < 0.01. b–e A human tissue array containing 40 lung carcinoma samples with paired lymphatic metastasis was analyzed for PRAK and HIF-1α expression by immunohistochemistry. Representative images (b) and H-scores for PRAK (c) and HIF-1α (d) staining are shown. Each symbol represents an individual sample. Bars are the mean and error bars represent s.e. **p < 0.01. The correlation of PRAK and HIF-1α expression was analyzed using R programming (e). f Kaplan–Meier survival analysis was performed with a cohort of 1926 lung cancer patients from a public database (http://kmplot.com/analysis/) to explore the correlation of survival time with PRAK expression. p-value was determined by two-tailed, unpaired t-test (a) and paired t test followed by Wilcoxon test (c, d).