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. 2021 Mar 19;4:368. doi: 10.1038/s42003-021-01914-8

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 6 — a PANC-1, CAPAN-1, and CFPAC-1 expressing ffLuc were cultured with NK cells with increasing doses of rIFNα. We also cultured NK cells expressing ffLuc with these cancer cells (E:T = 1:10) with increasing doses of rIFNα. Cells were harvested 0 and 72 h post coculture, and viable cells were analyzed by luciferase assay (n = 4 biologically independent samples, each timepoint). Data are presented as means ± SD. P-values were determined by ordinary one-way ANOVA with Tukey multiple comparisons. CFPAC (F(4,15) = 351.8) NK (F(4,15) = 346.8), Panc1 (F(4,15) = 468.7) NK (F(4,15) = 106.3), CAPAN1 (F(4,15) = 1121) NK (F(4,15) = 318.4. b PANC-1, CAPAN-1, and CFPAC-1 expressing ffLuc (or not expressing) were infected with 100 vp/cell of HDAdTrio. Cells expressing ffLuc were cultured with NK cells (E:T = 1:10). We also cultured NK cells expressing ffLuc with these cancer cells (E:T = 1:10) at 24 h post infection of HDAdTrio in the presence or absence of 1 ng/mL rIFNα. Cells were harvested 72 h post coculture, and viable cells were analyzed by luciferase assay (n = 4 biologically independent samples). Data are presented as means ± SD. P-values were determined by ordinary one-way ANOVA with Tukey multiple comparisons. CFPAC (F(3,12) = 1114) NK (F(3,12) = 458.3), Panc1 (F(3,12) = 356.6) NK (F(3,12) = 410.2), CAPAN1 (F(3,12) = 459.6) NK (F(3,12) = 131.4). c We sampled media 72 h post coculture, and measured granulysin using ELISA assay (n = 4 biologically independent samples). Data are presented as means ± SD. P-values were determined by ordinary one-way ANOVA with Tukey multiple comparisons, p < 0.001 (F(3,12) = 41.07), p < 0.0001 (F(3,12) = 74.40), p < 0.0001 (F(3,12) = 49.49). Statistical significance set at p < 0.05, ns > 0.05.