Skip to main content
. 2021 Mar 19;12:1750. doi: 10.1038/s41467-021-21955-1

Fig. 2. LMIV230-01 binds to multiple parasite stages and its activity is complement-dependent.

Fig. 2

a LMIV230-01 binds to permeabilized gametocytes, gametes, and zygotes and does not bind to ookinetes. Parasites were fixed and permeabilized, and 7.5 µg/mL of antibody was used to stain the different parasite stages. Scale bars: 5 µM. This experiment was performed in duplicate. b In vitro parasite lysis by LMIV230-01 is complement-dependent. Samples were tested in two independent assays, using two different parasite cultures. Data for LMIV230-02 mAb are shown in Supplementary Fig. 8a. c Functional activity of LMIV230-01 is also complement-dependent in vivo (SMFA with mosquitoes). Data from three independent SMFA assays. N ≥ 20 mosquitos per assay. Data for LMIV230-02 mAb are shown in Supplementary Fig. 8b. Oocyst averages in the control mosquitoes (fed with IgG1 targeting P. yoelli P140) for each of the experiments were: exp. 1 = 4.55; exp. 2 = 20.50, exp. 3 = 5.86. Data obtained from mosquitoes fed with LMIV230-01 at 1000 µg/mL with intact sera were also used to generate Fig. 1f. Values are shown as mean ± s.e.m. One-Way ANOVA and Tukey’s multiple comparisons test were used to compare the different groups. d Live imaging of P. falciparum NF54 female gametes incubated with LMIV230-01 in the presence of intact serum from a healthy donor revealed surface-deposited MAC (membrane attack complex) using anti-C5b-9 + C5b-8 antibody (magenta color). MAC deposition was not detected in the presence of heat-inactivated (HI) serum. Scale bars: 5 µM. This experiment was performed in triplicate. Source data are provided as a Source Data file.