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. 2021 Mar 19;12:1789. doi: 10.1038/s41467-021-22065-8

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 2 — a The expression of the indicated IFT components in the phasmid cilia and the soma of phasmid and amphid neurons of WT worms at indicated ages. The tested proteins were endogenously tagged with GFP using CRISPR/Cas9 technology. Scale bars: 5 μm. n > 20 animals in each experiment. Exact sample size and p value are included in Source Data file. b The expression of endogenously tagged DAF-19::GFP in the soma of phasmid and amphid neurons of WT worms at indicated ages. Scale bars: 5 μm. n > 20 animals in each experiment. Exact sample size and p value are included in Source Data file. c The expression of DAF-19c::mCherry in the soma of amphid neurons at indicated ages. Scale bar: 5 μm. n = 20 animals in each experiment. p = 0.5763. d The expression of IFT components in the phasmid cilia, phasmid neurons and amphid neurons in the indicated strains at day 1 and day 10 of adulthood. The worms overexpressing daf-19c were examined in parallel to the WT worms in A. Scale bar: 5 μm. n > 20 animals in each experiment. Exact sample size and p value are included in Source Data file. Data are presented as mean ± SD. Unpaired t-test (two-tailed), *p < 0.05, **p < 0.01, ***p < 0.001, ns non-significant. Source data are provided as a Source Data file.