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. 2021 Mar 19;11:6472. doi: 10.1038/s41598-021-85960-6

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Expression of nutrient transporters in human IL-12/15/18-stimulated NK cells. Bar charts representing (A) the percentage of control NK and IL-12/15/18-stimulated (activated NK or CIML NK at day 0 or 7, respectively) NK cells expressing the transferrin receptor CD71, and expression levels of (B) the heavy chain of multiple heterodimeric amino acid transporters CD98, and glucose transporters (C) GLUT1 and (D) GLUT3, measured as median fluorescence intensity (MFI). Graphs show data of cells after 16–18 h of IL-12/15/18-stimulation (Day 0, left column) and after 7 days of culture with IL-15 (control NK or CIML NK) or IL-2 (CIML NK + IL-2) (Day 7, right column). Means ± SEM are depicted. Statistical analyses were performed using Wilcoxon matched-pairs signed rank test. Each dot represents an independent experiment from a different donor (n = 6–9). *P < 0.05, **P < 0.01.