Skip to main content
. 2021 Mar 19;11:6472. doi: 10.1038/s41598-021-85960-6

Figure 5.

Figure 5

2-DG-induced inhibition of specific cytokine/chemokine production and degranulation of human cytokine-induced memory-like (CIML) NK cells at day 7. Control and activated NK cells cultured for seven days with IL-15 (control NK or CIML NK) or IL-2 (CIML NK + IL-2), were restimulated by co-culturing them with K562 target cells (E:T ratio = 1:1) or restimulated with IL-12, IL-15 and IL-18 (10, 100 and 50 ng/mL, respectively) for 7 h in the presence and absence of 50 mM 2-DG. Bar graphs showing the specific percentage of cells that produce IFNγ, TNF and MIP-1β, or degranulate (CD107a), in the presence and absence of 2-DG. Specific percentage of positive cells was calculated by subtracting the percentage of positive cells for each measured function in the absence of stimuli. Means ± SEM are depicted. Statistical analyses were performed using Wilcoxon matched-pairs signed rank test. n = 7–9. ns = non-significant, *P < 0.05, **P < 0.01.