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. 2021 Mar 19;11:6472. doi: 10.1038/s41598-021-85960-6

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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2-DG-induced inhibition of cytotoxic activity of human cytokine-induced memory-like (CIML) NK cells at day 7. Control and activated NK cells cultured for seven days with IL-15 (control NK or CIML NK) or IL-2 (CIML NK + IL-2), were co-cultured with calcein-AM-labeled K562 target cells at different E:T ratios for 3 h in the presence and absence of 50 mM 2-DG. Percentage of specific lysis was measured based on calcein-AM release. Means ± SEM are depicted. Statistical analyses were performed using Wilcoxon matched-pairs signed rank test. n = 7–8. ns = non-significant, *P < 0.05, **P < 0.01.